Top seed Vera Zvonareva battled past wildcard Serena Williams in a three set thriller 3-6 7-6 7-5.
The Russian star always held the upper hand in what became a baseline battle of attrition with neither player looking jolly on Centre Court.
It was a dour encounter, miserable-looking Williams seemed determined to fight from the back of the court and try to end points swiftly on her service games then wearing her opponent out on their serve.
Williams possessed the power strokes when she needed it but chased a ball all match, determined to preserve her energy.
Her tactics resulted in the opening set without breaking a sweat but she lost the second set on a tie-break 7-5 after taking a tumble on set point. Zvonareva’s array of weapons from the baseline carved out a 5-3 lead but the American superstar fought back to 5-5 before bowing out 7-5.